关键词: 子宫肿瘤, 子宫内膜癌, LKB1, 慢病毒

Abstract: Objective To investigate lentivirus-mediated LKB1 gene overexpression in HEC-1A endometrial cancer cells as a basis for future research. Methods The LKB1 gene was subcloned by RT-PCR from a cDNA plasmid into a lentiviral pWPI expression vector， generating the plasmid LKB1/pWPI．This plasmid was co-transfected into 293T cells together with the packaging plasmid pCMV-Dr8.74 and pMD2.G.The recombinant virus was used to infect HEC-1A cells，and LKB1 expression was measured using real-time quantitative PCR（qRT-PCR） and Western blotting. Results We succeeded in constructing the recombinant plasmid LKB1/pWPI and packaging it into lentiviral particles in 293T cells.This virus infected HEC-1A cells efficiently， leading to higher LKB1 expression than in the parental cells or untransfected controls（P<0.01）. Conclusion The LKB1 gene has been incorporated into a lentiviral expression vector， allowing studies of its effects on the development of endometrial cancer.

Key words: Uterine neoplasm, Endometrial cancer, LKB1, Lentivirus